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METHODS IN MOLECULAR BIOLOGY BACKUP |
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001 - Antisense Therapeutics |
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002 - Human Cell Culture Protocols |
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003 - New Protein Techniques |
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004 - Prion Diseases |
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005 - New Nucleic Acid Techniques |
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006 - Vaccine Protocols |
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007 - Animal Cell Culture |
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008 - Molecular Diagnosis of Genetic Diseases |
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009 - Plant Cell and Tissue Culture |
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010 - Lectin Methods and Protocols |
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011 - Protocols in Human Molecular Genetics |
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012 - Herpes Simplex Virus Protocols |
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013 - Immunochemical Protocols, 1st Edition |
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014 - Therapeutic Applications of Ribozymes |
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015 - Diagnostic Virology Protocols |
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016 - Pulsed-Field Gel Electrophoresis Protocols |
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017 - Protocols in Molecular Neurobiology |
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018 - Computer Analysis of Sequence Data |
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019 - Basic Cell Culture Protocols |
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020 - Immunochemical Protocols, 2nd Edition |
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021 - Methods in Genomic Neuroscience by Vdxiaozhang |
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MOLECULAR BIOLOGY-FIX |
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Antisense Therapeutics Second Edition |
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1. Antisense Therapeutics A Promise Waiting to Be Fulfilled |
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2. Antisense Inhibition Oligonucleotides, Ribozymes, and siRNAs |
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3. Local Application of Antisense for Prevention of Restenosis |
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4. Antisense Therapeutics for Hypertension Targeting the Renin Angiotensin System |
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5. Antisense Strategies for Treatment of Heart Failure |
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6. Clinical Studies of Antisense Oligonucleotides for Cancer Therapy |
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7. Antisense Therapy in Clinical Oncology Preclinical and Clinical Experiences |
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8. Radionuclide Peptide Nucleic Acid in Diagnosis and Treatment of Pancreatic Cancer |
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9. Suppression of Pancreatic and Colon Cancer Cells by Antisense K ras RNA Expression Vectors |
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10. Induction of Tumor Cell Apoptosis and Chemosensitization by Antisense Strategies |
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11. Utility of Antioncogene Ribozymes and Antisense Oligonucleotides in Reversing Drug Resistance |
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12. Transport of Antisense Across the Blood Brain Barrier |
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13. Transdermal Delivery of Antisense Oligonucleotides |
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14. Antisense Strategies for Redirection of Drug Metabolism Using Paclitaxel as a Model |
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15. Antisense Oligonucleotide Treatment of Inflammatory Bowel Diseases |
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Checkpoint Controls and Cancer Volume 1 Reviews and Model Systems |
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1. G1 and S-Phase Checkpoints, Chromosome Instability, and Cancer |
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2. Analyzing the G2M Checkpoint |
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3. Analyzing the Spindle Checkpoint in Yeast and Frogs |
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4. Cell Cycle Checkpoint Control Mechanisms That Can Be Disrupted in Cancer |
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5. Establishment of a Cell-Free System to Study the Activation of Chk2 |
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6. Analyzing Checkpoint Controls in Human Skin |
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7. Generation and Analysis of Brca1 Conditional Knockout Mice |
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8. Analysis of Cell Cycle Progression and Genomic Integrity in Early Lethal Knockouts |
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9. Xenopus Cell-Free Extracts to Study the DNA Damage Response |
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10. A Xenopus Cell-Free System for Analysis of the Chfr Ubiquitin Ligase Involved in Control of Mitotic Entry |
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11. Control of Mitotic Entry After DNA Damage in Drosophila |
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12. Methods for Analyzing Checkpoint Responses in Caenorhabditis elegans |
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13. Assaying the Spindle Checkpoint in the Budding Yeast Saccharomyces cerevisiae |
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14. Purification and Analysis of Checkpoint Protein Complexes From Saccharomyces cerevisiae |
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METHODS IN MOLECULAR BIOLOGY BACKUP |
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Checkpoint Controls and Cancer Volume 2 Activation and Regulation Protocols |
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1. Analysis of RB Action in DNA Damage Checkpoint Response |
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2. Interaction Between the Retinoblastoma Protein and Protein Phosphatase 1 During the Cell Cycle |
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3. Generation of p53 Target Database Via Integration of Microarray and Global p53 DNA-Binding Site Analysis |
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4. Functional Analysis of CDK Inhibitor p21 |
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5. Analysis of p21CDKN1A Recruitment to DNA Excision Repair Foci in the UV-Induced DNA Damage Response |
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6. Quantitative Determination of p16 Gene Expression by RT-PCR |
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7. Measuring Cyclin-Dependent Kinase Activity |
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8. CHK1 Kinase Activity Assay |
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9. Assaying Cdc25 Phosphatase Activity |
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10. Analyzing the Regulation and Function of ATM |
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11. Use of siRNA to Study the Function of MDC1 in DNA Damage Responses |
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12. Functional Analysis of APC-Cdh1 |
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13. Purification of the Mitotic Checkpoint Complex,an Inhibitor of the APCC From HeLa Cells |
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14. Analysis of the Spindle-Assembly Checkpoint in HeLa Cells |
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15. Analysis of Checkpoint Responses to Histone Deacetylase Inhibitors |
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16. Analysis of DNA Repair and Chromatin Assembly In Vitro Using Immobilized Damaged DNA Substrates |
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17. Analyzing Cell Cycle Checkpoints After Ionizing Radiation |
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18. FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells |
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19. Analysis of Cell Cycle by Flow Cytometry |
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20. Analyzing Markers of Apoptosis In Vitro |
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21. Analysis of Telomerase Activity and Telomere Function in Cancer |
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Differential Display Methodes and Protocol_2nd Edition |
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001. Silencing in Yeast. |
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002. Identification of mRNA Bound to RNA Binding Proteins by Differential Display |
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003. Automated Pattern Ranking in Differential Display Data Analysis |
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004. Automation of Fluorescent Differential Display With Digital Readout |
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005. Comprehensive Analysis of Ovarian Gene Expression During Ovulation Using Differential Display |
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006. Detection of an mRNA Polymorphismby Differential Display |
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007. Differentially Expressed Genes Associated With Hepatitis B Virus HBx and MHBs Protein Function in Hepatocellular Carcinoma |
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008. Functional Analysis of Nocturnin |
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009. GeneCalling® |
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010. Global Analysis of Gene Expression by Differential Display |
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011. High-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCR |
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012. Identification by Differential Display of IL-24 Autocrine Loop Activated by Ras Oncogenes |
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013. Identification of Disease Markers by Differential Display |
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014. Identification of p53-Regulated Genes by the Method of Differential Display |
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015. Identification of Target Genes of a Yeast Transcriptional Repressor |
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016. Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. crispa by Differential Display |
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017. Linking cDNA-AFLP-Based Gene Expression Patterns and ESTs |
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018. Ordered Differential Display |
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019. Saturation Screening for p53 Target Genes by Digital Fluorescent Differential Display |
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020. Vertical Arrays |
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DNA Viruses - Methods and Protocols |
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1. Viral Detection |
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2. Quantitative Detection of Epstein-Barr Virus DNA in Clinical Specimens by Rapid Real-Time PCR Targeting a Highly Conserved Region of EBNA-1 |
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3. Profiling of Epstein-Barr Virus Latent RNA Expression in Clinical Specimens by Gene-Specific Multiprimed cDNA Synthesis and PCR. |
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4. Quantitative Detection of Viral Gene Expression in Populations of Epstein-Barr Virus-Infected Cells In Vivo |
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5. Reporter Cell Lines for the Detection of Herpes Simplex Viruses |
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6. Unraveling the Architecture of Viruses by High-Resolution Atomic Force Microscopy |
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7. Studying the Structure of Large Viruses With Multiresolution Imaging |
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8. Herpes Simplex Virus Cell Interactions Studied by Low-Fading Contrasted Immunofluorescence |
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9. Herpes Simplex Virus Cell Interactions Studied by Immunogold Cryosection Electron Microscopy |
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10. FTIR Microscopy Detection of Cells Infected With Viruses |
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11. The JC Virus-Like Particle Overlay Assay |
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12. Analysis of Fusion Using a Virus-Free Cell Fusion Assay |
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13. Pseudovirions as Specific Tools for Investigation of Virus Interactions With Cells |
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14. Simultaneous In Situ Detection of RNA, DNA, and Protein Using Tyramide-Coupled Immunofluorescence |
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15. Identification and Characterization of Herpesviral Immediate-Early Genes |
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16. Methods for Measuring the Replication and Segregation of Epstein-Barr Virus-Based Plasmids |
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17. DNA Affinity Purification of Epstein-Barr Virus OriP-Binding Proteins |
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18. Pre-B-Cell Colony Formation Assay |
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19. Luciferase Real-Time Bioluminescence Imaging for the Study of Viral Pathogenesis |
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20. Culturing Primary and Transformed Neuronal Cells for Studying Pseudorabies Virus Infection |
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21. Human Papillomavirus Type 31 Life Cycle Methods for Study Using Tissue Culture Models |
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22. Molecular Genetics of Herpesviruses A Recombinant Technology`Approach |
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23. Molecular Genetics of DNA Viruses Recombinant Virus Technology |
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24. Genetic Analysis of Cytomegalovirus by Shuttle Mutagenesis |
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25. Construction of a Gene Inactivation Library for Bovine herpesvirus 1 Using Infectious Clone Technology |
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26. Selective Silencing of Viral Gene E6 and E7 Expression in HPV-Positive Human Cervical Carcinoma Cells Using Small Interfering RNAs |
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27. Real-Time Quantitative PCR Analysis of Viral Transcription |
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28. Rapid Screening of Chemical Inhibitors That Block Processive DNA Synthesis of Herpesviruses |
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Methods in Molecular Biology Backup 2 |
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001. Calcium Signaling Protocols |
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002. Calcium-Binding Protein Protocols. Volume 1 |
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003. Calcium-Binding Protein Protocols. Volume 2 |
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004. Confocal Microscopy |
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005. Dendritic Cell Protocols by XssX2000 |
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006. DNA Methylation Protocols |
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007. E. Coli Gene Expression Protocols |
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008. Embryonic Stem Cells Methods and Protocols |
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009. Flow Cytometry Protocols-second edition |
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010. Functional Genomics |
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011. Gene Knockout Protocols |
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012. Genomic Imprinting-Methods and Protocols |
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013. Genomics Protocols |
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014. Mitochondrial DNA-Methods and Protocols |
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015. PCR Cloning Protocols |
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016. Pcr protocols 2e |
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017. RT-PCR Protocols |
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018. Transgenic Mouse Methods and Protocols |
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019. Two Hybrid System-Methods and Protocols |
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020. CancerCellSignaling |
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021. Cytokine and Colony Stimulating Factor Methods and Protocols |
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022. Gene Targeting Protocols |
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023. In Vitro Mutagenesis Protocols |
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024. Membrane Protein Protocols |
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025. Methods in Molecular Biology(vol229,2003)-Lentivirus Gene Engineering Protocols |
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026. MHC Protocols |
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027. Oxidative Stress Biomarker-Antioxdative Protocols |
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028. PCR Mutation Detection Protocols |
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029. Single Nucleotide Polymorphisms-Methods and Protocols |
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030. The Elisa Guide Book |
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031. Transcription Factor Protocols |
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Oligonucleotide Synthesis - Methods and Applications |
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1. Synthesis of DNA Using a New Two-Step Cycle |
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2. RNA Synthesis Using 2′-O-(Tert-Butyldimethylsilyl) Protection |
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3. RNA Oligonucleotide Synthesis Via 5′-Silyl-2′-Orthoester Chemistry |
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4. Dimethylthiarum Disulfide New Sulfur Transfer Reagent in Phosphorothioates Oligonucleotide Synthesis |
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5. Assay for Evaluating Ribonuclease H-Mediated Degradation of RNA Antisense Oligonucleotide Duplexes |
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6. Di- and Oligonucleotide Synthesis Using H-Phosphonate Chemistry |
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7. Solid-Phase Synthesis of Circular Oligonucleotides |
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8. Locked Nucleic Acid Synthesis |
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9. Synthesis of DNA Mimics Representing HypNA pPNA Hetero-Oligomers |
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10. Chemical Methods for Peptide Oligonucleotide Conjugate Synthesis |
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11. Biotin-Labeled Oligonucleotides With Extraordinarily Long Tethering Arms |
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12. Universal Labeling Chemistry for Nucleic Acid Detection on DNA Chips |
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13. Postsynthetic Functionalization of Triple Helix-Forming Oligonucleotides |
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14. Fluorescence-Based On-Line Detection as an Analytical Tool in RNA Electrophoresis |
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15. Design and Optimization of Molecular Beacon Real-Time Polymerase Chain Reaction Assays |
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16. High-Throughput Production of Optimized Primers (Fimers) for Whole-Genome Direct Sequencing |
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17. Nonenzymatic Template-Directed RNA Synthesis |
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18. DNase I Footprinting of Small Molecule Binding Sites on DNA |
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19. Gene Targeting Using Peptide Nucleic Acid |
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20. Nucleic Acid Library Construction Using Synthetic DNA |
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21. In Vitro Selection From Combinatorial Nucleic Acid |
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22. In Vitro Selection Procedures for Identifying DNA |
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23. Short Double-Stranded Ribonucleic Acid as Inhibitor of Gene Expression by the Interference Mechanism |
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Peptide Synthesis and Applications |
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1. Fundamentals of Modern Peptide Synthesis |
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2. Chimerism A Strategy to Expand the Utility and Applications of Peptides |
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3. Modification of Peptides and Other Drugs Using Lipoamino Acids and Sugars |
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4. Synthesis of Linear, Branched, and Cyclic Peptide Chimera |
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5. Synthesis of Cell-Penetrating Peptides for Cargo Delivery |
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6. Incorporation of Phosphotyrosyl Mimetic 4-(Phosphono difluoromethyl) phenyl alanine (F2Pmp) Into Signal Transduction-Directed Peptides |
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7. Expressed Protein Ligation for Protein Semisynthesis and Engineering |
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8. Cellular Delivery of Peptide Nucleic Acid by Cell-Penetrating Peptides |
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9. Quenched Fluorescent Substrate-Based Peptidase Assays |
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10. A Convenient Method for Synthesis of Cyclic Peptide Libraries |
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11. High-Throughput Peptide Synthesis |
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12. Backbone Amide Linker Strategies for the Solid-Phase Synthesis of C-Terminal Modified Peptides |
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13. Synthesis of Peptide Bioconjugates |
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14. Protein Identification by Mass Spectrometric Analyses of Peptides |
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15. Manual Solid-Phase Synthesis of Glutathione Analogs A Laboratory-Based Short Course |
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Unsorted |
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001. Calcium Signaling Protocols |
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002. Calcium-binding Protein Protocols. Volume 1 |
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003. Calcium-binding Protein Protocols. Volume 2 |
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004. Confocal Microscopy |
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005. Current Protocols in Protein Science |
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006. DNA Methylation Protocols |
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007. E. Coli Gene Expression Protocols |
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008. Embryonic Stem Cells Methods and Protocols |
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009. Flow Cytometry Protocols-Second Edition |
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010. Gene Knockout Protocols |
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011. Genomic Imprinting-Methods and Protocols |
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012. Genomics Protocols |
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013. Mitochondrial DNA-Methods and Protocols |
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014. PCR Cloning Protocols |
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015. Pcr protocols 2e |
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016. RT-PCR Protocols |
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017. Telomeres and Telomerase Methods and Protocols |
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018. Transgenic Mouse Methods and Protocols |
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019. Two Hybrid System-Methods and Protocols |
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020. Basic Cell Culture Protocols(1) |
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021. CancerCellSignaling |
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022. Curent protocols in Cytometry |
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023. Cytokine and Colony Stimulating Factor Methods and Protocols |
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024. DNA Array Method and Protocols |
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025. DNA Methylation Protocols |
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026. DNA-Protein Interactions |
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027. E.Coli Gene Expression Protocols |
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028. Embryonic Stem Cells Methods and Protocols |
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029. Flow Cytometry Protocols |
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030. Functional Genomics Methods and Protocols |
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031. Gene Knockout Protocols |
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